jy9202 Veľmi pokročilý
Počet príspevkov : 542 Registration date : 18.12.2013
| Predmet: Cells were lysed and immunoprecipitated with either anti Flag M2 beads or HA an Št január 02, 2014 5:57 am | |
| In cancer cells, p53 protein is marked for proteasomal degradation by mdm2 protein, a E3 type of ubiquitin ligase, Mdm2 ligase is found to be highly expressed in cancers, though the underlying mechanism is still unclear, possibly to keep p53 protein away from JAK3 阻害剤 tumor formation processes, Proteasomal inhibition allows accumulation of p53 and its nuclear ex port in cancer cells and thereby increase the expression of its transcriptional target gene p21. p21, a potent CDK inhibitor binds and inactivate cyclin E and the CDK2 complex. This complex is essential for late G1 phase of cell cycle mediating entry in the S phase of cell cycle, However activation of p53 and p21 proteins Cyclin D expression and thus regulating the G1 S cell cycle arrest.<br><br> MG 132 treatment significantly increased supplier LDE225 the phosphorylation of p38 MAPK in Calu 6 cells and its inhibition partially inhibited MG 132 induced cell death, Lastly, c Jun N terminal Kinase, the third component of the MAPK pathway, has been reported to be activated in MG 132 induced apoptosis, Bortezo mib treatment activated JNK in glioblastma multiforme cells causing G2 M arrest with subsequent upre gulation of p21 and p27 proteins and simultaneous de crease in cyclin B, CDK2, CDK4 and E2F4 protein levels, More recently Bortezomib has also been reported to activate JNK in head and neck cancer cells, JNK1 binds and phosphorylates E2F and limit its ability to bind to DNA, this may induce G1 arrest subsequent to CDK mediated phosphorylation of pRb, On the other hand its activation leads to cell proliferation by increasing the expression of cyclin D1 and repression of p53 and p21 proteins, However correlation of JNK to the cell cycle arrest is still a matter of investiga tion.<br><br> Involvement of MAPK pathways in cell cycle and PI induced cell death pave the way for identification of many of the novel targets LY2157299 TGF-beta 阻害剤 which are supposed to provide a mechanistic link between these two processes, p53 21 pathway: blocking the doorstep of cell cycle entry Transcription factor p53 lies in very close association with both proteasome and cell cycle pathways, It leads to G0 G1 cell cycle arrest but probably there are several other mediators of this pathway regulating PI in duced G0 G1 arrest. Rb E2F pathway: regulating DNA synthesis Rapid proteasomal degradation of the retinoblastoma protein is evident in several cancers.<br><br> Many oncoproteins are engaged in triggering ubiquitin proteasome degra dation of Rb including viral oncoprotein E7 of human pappiloma virus type 16, Epstein Barr virus nuclear anti gen 3C and gankyrin, Rb act as a tu mor suppressor protein playing crucial role in cell cycle regulation, DNA replication, DNA damage repair and many other cellular processes. The preliminary role of Rb in cell cycle regulation is binding and stabilization of E2F family of proteins causing their transcriptional re pression. Transcriptionally repressed E2F becomes inef fective in activating genes required for DNA synthesis and S phase progression, Administration of PIs me diates Rb protein escape from proteasomal degradation. Non degraded Rb still remains bounded to E2F which indirectly inhibits E2F mediated expression of S phase progression genes thereby arresting cancer cells in G0 G1 or S phase, According to a recent study, Bortezomib induced cell cycle arrest and apoptosis in BCR Abl expressing, imatinib resistant and sensitive CML cells. | |
|