In the confirmatory clinical trials for Kalydeco, a treatment for patients with

The solvent was removed to yield a brown solid which was triturated with boiling 3060 C petroleum ether, cooled and MAPK 検定 collected. This solid was further washed with 3060 C petroleum ether and dried to yield crude N tert butoxycarbonyl 4 aniline as a pale brown solid. The crude protected aniline was dissolved in dichloromethane then treated with trifluoroacetic acid and stirred at ambient temperature for about 3 hours. Water was added and the aqueous acidic layer was separated. The remaining organic layer was further extracted with aqueous hydrochloric acid. The combined acidic aqueous layers were washed with dichloromethane, cooled with ice then basified by the addition of solid sodium hydroxide while maintaining a temperature of below 15 C.<br><br> The MK-1775 溶解度 resulting aqueous layer was extracted with ethyl acetate and the combined organic layers were washed with water, dried over anhydrous magnesium sulphate, filtered and concentrated to yield a mauve solid. This was crystallized from ethyl acetate and 3060 C petroleum ether, filtered, washed with 3060 C petroleum ether and dried to yield 4 aniline as a pale mauve powdery solid using a Vydac Genesis C8 column with diode array, evaporative light scattering and positivenegative electrospray ionization detection Rt2. 43 min. MS mz 254. Step b. N 3 chloro benzenesulfonamide 3 Chlorobenzenesulfonyl chloride was added to a mixture of 4 aniline in 1,4 dioxane and N,N diisopropylethylamine. The resulting mixture was stirred at ambient temperature for about 16 hours then dispensed into a microwaveable tube and anhydrous hydrazine was added.<br><br> The tube was sealed and heated at 140 C for 15 minutes in a microwave. The solvent was removed under reduced pressure and the residue was purified by reverse phase preparative chromatography followed by chromatography over silica gel using a mixture of 91 dichloromethanemethanol as the eluent to afford N 3 chloro benzenesulfonamideS using a Zorbax XDB C18 column with diode array, evaporative ms-275 分子量 light scattering and positivenegative electrospray ionization detection Rt2. 32 min. MS mz 399, 401. Furan 2 carboxylic acid 5 1 H indazol 3 ylamide 2 Furoyl chloride was added dropwise to a solution of N 3 chloro benzenesulfonamide in pyridine at about 0 C. The reaction was allowed to warm to ambient temperature and stirred for 18 hours.<br><br> Ethyl acetate and methanol were added to the reaction mixture and the resulting solution was washed with water. The organic layer was dried over anhydrous magnesium sulphate and concen trated under reduced pressure. The residue was purified over silica gel eluting with a gradient of 0100% ethyl acetate in heptane to afford furan 2 carboxylic acid 5 1 H indazol 3 ylamide. 1 H NMR using a Zorbax XDB C18 column with diode array, evaporative light scattering and positivenegative electrospray ionization detection Background Patient reported outcome is an umbrella term used to describe data collected directly from the patient without interpretation by clinicians or others. PRO data are collected using standardized questionnaires, diaries, or event logs that are designed to measure an explicit concept, such as signs and symp toms of disease, functioning, health statushealth related quality of life, and treatment satisfaction.

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