jq123 Začiatočník
Počet príspevkov : 93 Registration date : 14.04.2015
| Predmet: 0 Expression BeadChip kit following the producer rec ommend Ut september 29, 2015 5:41 am | |
| Indentation force curves of DSPC monolayer movie have been obtained at differ ent sites on the film without the need of defect, and an instance of indentation force curve is described in Figure 2B exactly where there is a yield threshold denoted by a black arrow. The break by way of force at the yield level is 0. 85 0. 52 nN and penetration distance is two. 24 1. 01 nm counted from Ivacaftor 価格 510 curves. This statistical penetration distance of the DSPC mono layer movie calculated from indentation force curves represents the thickness with the film, that's in agree ment with that of DSPE monolayer in water measured by modified AFM probe. On top of that, the break as a result of force on the yield threshold as well as measured thickness values with AFM are relevant to sev eral experimental parameters, this kind of as the cantilever spring continuous, the shape and size of the used AFM tip.<br><br> LDE225 smoothened 拮抗薬 These outlined parameters are diverse from one another truly, which final results in substantial error ranges. In contrast with the data ranging involving 4nN to 10 nN, the error ranges of our break force and penetration distance are acceptable based to the measurements of 200 or 510 force curves. As a way to validate the indentation force curves of DSPC monolayer LB movie, monolayer film of another phospholipid was researched together with the same system as that of DSPC monolayer film in each sample preparation and sample characterization. DLPC has the same simple molecular construction as that of DSPC. DLPC has 12 repeat carbon units while DSPC has 18 repeat carbon units, and then the length of DLPC molecule is shorter than that of DSPC molecule.<br><br> Then the layers with the two phospholipids have been expected to be of distinct mechanical properties at nano Newton degree. AFM experiment LY2109761 dissolve 溶解度 outcomes of DLPC are expressed in Figure 3A and Figure 3B. The defect while in the topography image of DLPC monolayer movie was applied to distinguish the film through the substrate topography, and the indentation force curves were collected at various internet sites in the film. The indenta tion force curve from the movie in AFM tip approaching approach is similar to that of DSPC, in which there may be also a yield threshold marked by a black arrow. The break by means of force with the yield stage is 0. 41 0. 26 nN and penetration distance is 2. 33 0. 87 nm counted from 510 curves.<br><br> The statistical yield force of DLPC monolayer is smaller sized than that of DSPC monolayer, which states that DLPC monolayer, composed of mole cules with shorter alkyl tails, is weaker than that of DSPC mono layer. In other words, DSPC monolayer is more difficult to become penetrated. It will likely be uncovered the penetration distances of phospholipid movies are smaller than that of filmstheor etical thickness, which is mainly due to the deformation of the phospholipid molecules once they are interacting with AFM tip during the penetrating procedure in our experiment. Moreover, the deformation of DSPC movie would be various from that of DLPC movie below experimental temperature of 28 C when their phase transition temperature are con sidered, which can be helpful in understanding the experiment result that no clear penetration distance big difference had been ob tained in between DSPC and DLPC. | |
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