Statistical analysisa significantly different from the control

The results indicated that GnRH I and GnRH II increased the invasive capacity of B6Tert 1 cells, with the invasion index increasing to 1. 6 and 1. 8 fold of control cultures, respectively. This observation is consistent with our previous report that GnRH I and GnRH II promote EVT cell invasion. oral JAK 阻害剤 The expression of mRNA for MMP 2, MMP 9, MMP 14, TIMP 1, TIMP 2 and TIMP 3 has been shown in B6Tert 1 cells, while MMP 26 expression has yet to be identified in this cell line. Western blotting analyses revealed that the expression level of MMP 26 protein in B6Tert 1 cells was comparable to that in the primary CTB cells at 6 7 gestational weeks, but much higher than that of human choriocarcinoma JEG 3 cells. These results suggest that the B6Tert 1 cell line is a valuable in vitro model to study the regulation of GnRH I and II on MMP 26 expression in trophoblast liketrophoblast cell.<br><br> Time dependent effects of GnRH I and GnRH II on MMP 26 expression in B6Tert 1 cells To investigate the regulatory effects of GnRH I and GnRH II on MMP 26 expression in human cytotropho blasts, mRNA LDE225 構造 and protein levels were analyzed from B6Tert 1 cells collected at various time points after treatment with GnRH I or GnRH II. Treat ment with GnRH I or GnRH II resulted in an increase in MMP 26 production at mRNA or protein level in a time dependent manner, whereas the addition of vehicle to the culture medium had no effect on the expression of MMP 26 in B6Tert 1 cells at any of the time points examined in this study. GnRH I increased the expression of MMP 26 mRNA in B6Tert 1 cells during the interval of 12 h to 48 h after treatment.<br><br> The levels of MMP 26 mRNA at 48 h were approximately 5 fold higher than that of the 0 h. A coordinate increase in the levels of MMP 26 protein in these cell cultures was also observed between 12 h and 48 h after GnRH I. GnRH II stimulated MMP 26 expression in B6Tert 1 cells in a manner differential to that of GnRH purchase LY2157299 Ilevels for MMP 26 mRNA and protein increased at 3 h after GnRH II treatment, peaked at 6 h, and gradually decreased thereafter. By 48 h after treatment, MMP 26 mRNA expression decreased to a level comparable to 0 h, while the protein level remained significantly greater than those detected at 0 h. Different dosage effects of GnRH I and GnRH II on MMP 26 production in B6Tert 1 cells To further determine the dosage effects of GnRHs on MMP 26 production, B6Tert 1 cells were treated with GnRH I or GnRH II at concentrations of 1, 10, 100 nM and 1 uM for 24 h.<br><br> GnRH I significantly increased MMP 26 mRNA and protein levels in B6Tert 1 cells at all the hormone concentrations examined in these stu dies. The treatment of 1 nM GnRH I was sufficiently high to induce maximal increase in MMP 26 expression. GnRH II increased MMP 26 mRNA and protein levels in B6Tert 1 cells in a dose dependent manner. A significant increase in MMP 26 expression was first detected at concentration of 10 nM and the highest expression of MMP 26 was observed in B6Tert 1 cells in the presence of 100 nM GnRH II. However, GnRH II at concentration of 1 uM was ineffective.