ATM is involved in the sequential post translational modification of NEMO

In addition, the increase of the anti apoptotic protein Bcl XL more than the pro apopto tic protein Bax in hypoxia, confirmed that this survival can be partially due to the inhibition of apoptosis. Inter estingly, no differences オーダー AP24534 in GM CSF release were observed in eosinophils cultured in normoxia vs. hypoxia. This data would suggest that the increased viabi lity of the cells is not a consequence of increased GM CSF production by hypoxic eosinophils. Second, we found that hypoxia increases the release of the most important pro angiogenic factor VEGF from eosinophils. VEGF is the key mediator of angiogenesis, being a specific mitogen for endothelial cells. Many cells, including structural cells such as endothelial cells and inflammatory cells, release VEGF as a response to hypoxia.<br><br> purchase AT7519 Interestingly, this effect was enhanced by the presence of GM CSF in a seemingly synergistic fashion. In addition, the release of IL 8, which stimulates endothelial cell proliferation and capil lary tube organization, was also found to be aug mented in eosinophils under hypoxia. It is important to point out that in the time point in which cytokine release was evaluated, no significant differences in cell viability were observed. Finally, we demonstrated for the first time that eosino phils express HIF 1, and that its subunit HIF 1a is stabi lized after a short exposure to hypoxic conditions in these cells. Based on the literature on HIF 1a induction, it may be concluded that the classical stabilization mechanism induced by hypoxia causes the up regulation of HIF.<br><br> In addition in our study, HIF 1a up regulation was further enhanced in the presence of GM CSF. This effect of GM CSF seems to correlate to its increasing effect on VEGF release. In assays performed after o. n. incubation, HIF 1a levels were found to be still high. In eosinophils, GM CSF induced ERK12 phosphory lation is involved pan Akt 阻害剤 in their migration and degranulation. MAPK cascade is known to participate in cell proliferation, differentiation, survival, and locomotion. In addition, ERK12 has a specific role in regulat ing VEGF gene expression. Richard et al. showed in fibroblasts that HIF 1a is strongly phos phorylated by ERK12 and that this is sufficient to pro mote the transcriptional activity of HIF 1 on VEGF. In the current study we showed that inhibition of ERK12 phosphorylation decreased both GM CSF induced HIF 1a and VEGF up regulation in eosino phils.<br><br> Interestingly, although no effect of hypoxia on ERK12 phosphorylation could be detected, PD98059 addition decreased both ERK12 and VEGF in eosinophils, to levels comparable to normoxia. This may be due to the multiple roles and interactions of ERK signaling. In summary, GM CSF increases HIF 1a levels and VEGF release by inducing ERK12 phosphorylation in a hypoxia independent way. We suggest that GM CSF might render the eosinophils more responsive to hypoxia induced activation by enhancing MAPK phos phorylation, which in turn contributes to HIF 1 stabilization. The present data showing cooperation between hypoxia and GM CSF on eosinophil function might be very relevant to what happens in vivo in allergic inflamed tissues, in which high levels of GM CSF and various levels of hypoxia frequently coexist.